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gentlemacs octo dissociator  (Miltenyi Biotec)
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Miltenyi Biotec gentlemacs octo dissociator
Individual hearts were excised from postnatal day 2 rat pups and ventricular tissue digested using an <t>Octo</t> <t>Dissociator</t> and Kit. Neonatal rat ventricular myocytes were cultured in 10% newborn calf serum for 48 hours prior to overnight serum depletion (16-18 hours). Cultured myocytes were treated with vehicle and 0.3 μM doxorubicin (Dox) for 24 hours. Filter-aided sample preparation (FASP) was performed on 25 µg cell lysates for data-independent acquisition (DIA) mass spectrometry. Image created with Biorender.
Gentlemacs Octo Dissociator, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gentlemacs octo dissociator/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
gentlemacs octo dissociator - by Bioz Stars, 2026-05
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heaters  (Miltenyi Biotec)
97
Miltenyi Biotec heaters
Individual hearts were excised from postnatal day 2 rat pups and ventricular tissue digested using an <t>Octo</t> <t>Dissociator</t> and Kit. Neonatal rat ventricular myocytes were cultured in 10% newborn calf serum for 48 hours prior to overnight serum depletion (16-18 hours). Cultured myocytes were treated with vehicle and 0.3 μM doxorubicin (Dox) for 24 hours. Filter-aided sample preparation (FASP) was performed on 25 µg cell lysates for data-independent acquisition (DIA) mass spectrometry. Image created with Biorender.
Heaters, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heaters/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
heaters - by Bioz Stars, 2026-05
97/100 stars
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Individual hearts were excised from postnatal day 2 rat pups and ventricular tissue digested using an Octo Dissociator and Kit. Neonatal rat ventricular myocytes were cultured in 10% newborn calf serum for 48 hours prior to overnight serum depletion (16-18 hours). Cultured myocytes were treated with vehicle and 0.3 μM doxorubicin (Dox) for 24 hours. Filter-aided sample preparation (FASP) was performed on 25 µg cell lysates for data-independent acquisition (DIA) mass spectrometry. Image created with Biorender.

Journal: bioRxiv

Article Title: Sex differences in cardiomyocyte proteomic responses to the cardiotoxic chemotherapy drug doxorubicin

doi: 10.64898/2026.04.21.715711

Figure Lengend Snippet: Individual hearts were excised from postnatal day 2 rat pups and ventricular tissue digested using an Octo Dissociator and Kit. Neonatal rat ventricular myocytes were cultured in 10% newborn calf serum for 48 hours prior to overnight serum depletion (16-18 hours). Cultured myocytes were treated with vehicle and 0.3 μM doxorubicin (Dox) for 24 hours. Filter-aided sample preparation (FASP) was performed on 25 µg cell lysates for data-independent acquisition (DIA) mass spectrometry. Image created with Biorender.

Article Snippet: Each C Tube containing ventricle tissue and 2.5 mL enzyme master mix was placed onto the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec, #130-096-427) and digested using the 37C_mr_NHDK_1 program.

Techniques: Cell Culture, Serum Depletion, Sample Prep, Data-independent acquisition, Mass Spectrometry